The c.1464C>T variant in MYOC is a synonymous variant (p.Ala488=). The highest minor allele frequency of this variant was in the East Asian population of gnomAD (v2.1.1) = 0.001253, which met the ≥ 0.001 threshold set for BS1 (25 alleles out of 19,950), meeting the threshold of ≥ 5 of at least 2,000 observed alleles). Although this synonymous variant was not predicted to affect splicing, as assessed with SpliceAI (≤ 0.2), it had a CADD score (v1.6) = 10.96, which did not meet the ≤ 10 threshold for BP4 and a GERP score = 2.87 (threshold < 0), not meeting BP7 and indicating conservation at this site. There was no functional evidence predicting a damaging or benign impact of this variant on MYOC function. As BS1 was met, PP1 did not apply and segregations were not counted. Although probands with primary open angle glaucoma have been reported carrying this variant, PM2_Supporting was not met, therefore PS4 did not apply. In summary, this variant met the criteria to receive a score of -4 and to be classified as likely benign (likely benign classification range -2 to -6) for primary open angle glaucoma based on the ACMG/AMP criteria met, as specified by the ClinGen Glaucoma VCEP (v1, 12 Oct 2021): BS1.