The c.752T>C variant in MYOC is a missense variant predicted to cause substitution of Valine by Alanine at amino acid 251 (p.Val251Ala). This variant was not found in any genetic ancestry group of gnomAD (v4.1.0), meeting the ≤ 0.0001 threshold set for PM2_Supporting in a genetic ancestry group of at least 10,000 alleles. The REVEL score = 0.839, which was within the 0.773-0.931 range for PP3_Moderate, predicting a damaging effect on MYOC function. There was no functional evidence predicting a damaging or benign impact of this variant on MYOC function. 17 segregations in 3 families, with juvenile or primary open angle glaucoma (JOAG or POAG), have been reported (PMIDs: 32937162, 23517641 and Pasutto pers. comm.), which fulfilled PP1_Strong (≥7 meioses in >1 family). 3 probands with JOAG or POAG have been reported (PMIDs: 32937162, 23517641 and Pasutto pers. comm.) carrying this variant, which met PS4_Supporting (≥ 2 probands). One of these was a confirmed de novo proband with juvenile open angle glaucoma (PMID: 23517641) (PS2_Moderate). In summary, this variant met the criteria to receive a score of 10 and to be classified as pathogenic (pathogenic classification ≥ 10, adapted from PMID: 32720330) for juvenile open angle glaucoma based on the ACMG/AMP criteria met, as specified by the ClinGen Glaucoma VCEP (v2.0.0, 5 Dec 2024): PP1_Strong, PS2_Moderate, PP3_Moderate, PM2_Supporting, PS4_Supporting