The NM_000070.3: c.1342C>T variant in CAPN3 is a missense variant that results in the substitution of arginine with cysteine at codon 448, p.(Arg448Cys). This variant has been identified in at least nine individuals with clinical features consistent with limb-girdle muscular dystrophy (LGMD) (PMID: 37852290, 39733240, 16141003, 37526466, 17318636, 27447704, 18854869, 15221789, 17236769; GRASP-LGMD Consortium internal data communication), including in a homozygous state in at least four patients without reported familial consanguinity (1.0 pts; PMID: 16141003, 37526466, 17318636, 27447704, 18854869, 17236769). It has also been identified in unconfirmed phase with a pathogenic CAPN3 variant in at least two patients (c.550del p.(Thr184ArgfsTer36), 0.5 pts, PMID: 39733240; c.1115+5G>C, 0.5 pts, GRASP-LGMD Consortium internal data communication) (PM3_Strong). At least one patient homozygous for this variant had both progressive limb girdle muscle weakness and absent calpain-3 protein expression in skeletal muscle, which is highly specific for CAPN3-associated LGMD (PMID: 18854869, 15221789; PP4_Strong). The highest frequency for this variant in gnomAD v4.1.0 is 0.00003412 (2/58616 Admixed American chromosomes), which is lower than the ClinGen LGMD VCEP threshold of <0.0001 for PM2_Supporting, meeting this criterion (PM2_Supporting). The computational predictor REVEL gives a score of 0.846, which is above the LGMD VCEP threshold of ≥0.70, evidence that correlates with impact to CAPN3 function (PP3). In addition, another missense variant at the same codon, c.1343G>A p.(Arg448His), has been classified as pathogenic by the ClinGen LGMD VCEP (PM5). In summary, this variant meets criteria to be classified as Pathogenic for autosomal recessive limb-girdle muscular dystrophy based on the ACMG/AMP criteria applied, as specified by the ClinGen LGMD VCEP (version 2.0.0; 01/20/2026): PM3_Strong, PP4_Strong, PM2_Supporting, PP3, PM5.