The NM_000023.4: c.241C>T variant in SGCA is a missense variant predicted to cause substitution of arginine by cysteine at amino acid 81 (p.Arg81Cys). This variant has been detected in at least three individuals with symptoms of limb girdle muscular dystrophy, including confirmed in trans with a pathogenic or likely pathogenic variant (c.850C>T p.(Arg284Cys), 1.0 pt, PMID: 17994539) and in a homozygous state in two patients (0.75 pts, PMID: 31069529, 30345904) (PM3). At least one patient with this variant and a second presumed diagnostic SGCA variant displayed progressive limb girdle muscle weakness and significantly reduced or absent alpha-sarcoglycan protein expression, which is highly specific for SGCA-related LGMD (PP4_Strong; PMID: 17994539, 26404900; LOVD Individual #00133411). In addition, the variant has been reported to segregate with autosomal recessive limb girdle muscular dystrophy in one affected family member (PP1; PMID: 17994539). The highest population minor allele frequency in gnomAD v2.1.1 is 0.00003 (1/30576 alleles) in the South Asian population, which is lower than the ClinGen LGMD VCEP threshold (0.00009) for PM2_Supporting, meeting this criterion (PM2_Supporting). The computational predictor REVEL gives a score of 0.934, which is above the threshold of 0.7, evidence that correlates with impact to SGCA function (PP3). In summary, this variant meets the criteria to be classified as Likely Pathogenic for autosomal recessive limb girdle muscular dystrophy based on the ACMG/AMP criteria applied, as specified by the ClinGen LGMD VCEP (LGMD VCEP specifications version 1.0.0; 01/09/2025): PP4_Strong, PM3, PP1, PP3, PM2_Supporting.