NM_001034853.2(RPGR):c.1753G>A (p.Glu585Lys) is a missense variant located in the final nucleotide of exon 14, and encodes the substitution of glutamic acid with lysine at amino acid 585. The splicing impact predictor SpliceAI gives a score of 0.52 for donor gain, which is above the ClinGen X-linked IRD VCEP recommended threshold of ≥0.2 and predicts a damaging impact on splicing. In vitro splicing studies confirm that the variant disrupts splicing of RPGR ORF15 and causes loss of transcript heterogeneity (PMID: 32788070). While these in silico and experimental findings would normally be counted under the PP3 and PS3_Supporting codes, respectively, they have been counted in combination as evidence of a loss-of-function effect (PVS1_RNA). This variant is absent from hemizygous individuals in gnomAD v4.1.0 (PM2_Supporting). At least one proband harboring this variant exhibits a phenotype including onset in first decade of life (1 pt), decreased visual acuity (0.5 pts), night blindness (0.5 pts), severe, diffuse, granular appearance and atrophy of retinal pigment epithelium (0.5 pts), bone-spicule pigmentation (0.5 pts), and arteriolar attenuation in mid-peripheral retina (0.5 pts), with genotyping by previous exome sequencing (2 pts), which together are highly specific for RPGR-related retinopathy (5.5 points, PMID: 32788070, PP4). In summary, this variant is classified as pathogenic for RPGR-related retinopathy based on the ClinGen X-linked Inherited Retinal Disease Expert Panel Specifications to the ACMG/AMP Variant Interpretation Guidelines for RPGR Version 1.0.0: PVS1_RNA, PM2_Supporting, and PP4. (date of approval 05/16/2025).