The c.1448G>A variant in ITGB3 is a missense variant predicted to cause substitution of Cysteine by Tyrosine at amino acid 483 (p.Cys483Tyr). This variant was identified in a patient (Patient 11, PMID: 2180491) with GT type 1. This patient had a bleeding phenotype, normal platelet count and morphology, absent or severely diminished platelet aggregation by adenosine diphosphate and other agonists but was normal by ristocetin. Another study (PMID 12152649) sequenced ITGB3 in this patient and identified this variant and demonstrated minimal expression on the cell surface using flow cytometry (PP4_Moderate). This same patient is also reported in PMIDs 28395882 and 3535160. This variant was found in the homozygous state in this GT patient in PMID: 12152649 (PM3_Supporting). COS-7 cells were transfected to express this variant and the wild-type alpha-2-beta-3 surface proteins. Both flow cytometry and immunoblot were used to measure the surface expression of proteins and demonstrated a greatly reduced surface expression, estimated from Figure 3 as 6.5% (PMID: 12152649; PS3_Moderate). This variant has a REVEL score of 0.948 which is above the threshold of 0.7 established by the PD EP (PP3). This variant is not present in the gnomAD database v4.1 (PM2_Supporting). In summary, this variant meets the criteria to be classified as Likely Pathogenic for autosomal recessive Glanzmann Thrombasthenia based on the ACMG/AMP criteria applied, as specified by the ClinGen PD VCEP: PP4_Moderate, PM3_Supporting, PS3_Moderate, PP3 and PM2_Supporting (VCEP specifications version 2.1.0).