The c.3658_3660del (p.Glu1220del) variant in MYH7 has been reported in 2 individuals with Ebstein anomaly (PS4_Supporting; Postma 2010 PMID:21127202; Bettinelli 2013 PMID 23956225; Partners LMM ClinVar SCV000059512.5). This variant segregated with Ebstein anomaly in 3 affected individuals from one family (Partners LMM ClinVar SCV000059512.5). While the expert panel waived the ACMG/AMP recommendation for demonstrating segregation in more than one family given that MYH7 is a well-established cardiomyopathy gene, its role in Ebstein anomaly is less established. Therefore, the expert panel felt that the PP1 pathogenic code should not be applied in this case given that all segregations came from one family. This variant was absent from large population studies (PM2; http://gnomad.broadinstitute.org). This variant is a deletion of 1 amino acid at position 1220 and is not predicted to alter the protein reading-frame. Given that only 1 amino acid has been deleted, the expert panel felt that adjusting to supporting evidence would be more appropriate in this case (PM4_Supporting). In summary, this variant is classified as uncertain significance for Ebstein anomaly in an autosomal dominant manner. MYH7-specific ACMG/AMP criteria applied (Kelly 2018 PMID:29300372): PM2; PM4_Supporting; PS4_ Supporting.