The NM_000527.5 (LDLR):c.-36T>G variant is classified as Uncertain significance – insufficient evidence for Familial Hypercholesterolemia by applying ACMG/AMP evidence codes PM2, PP4 and BS3_Supporting as defined by the ClinGen Familial Hypercholesterolemia Expert Panel LDLR-specific variant curation guidelines (specification version 1.2) on 28 October 2024. The supporting evidence is as follows: PM2: This variant is absent from gnomAD (gnomAD v4.1.0). PP4: Variant meets PM2, and is identified in one index case who fulfils DLCN criteria for probable FH after alternative causes of high cholesterol were excluded, reported in PMID 21538688 (Supplementary Table 2) by De Castro-Oros et al, 2011 from Universidad de Zaragoza, Spain. BS3_Supporting: Level 3 assay performed with luciferase reporter gene assay in HepG2 cells and there was no significant deference observed in gene expression comparing to wild-type construct, reported in PMID 21538688 by De Castro-Oros et al, 2011 from Universidad de Zaragoza, Spain. Level 3 assay performed with luciferase reporter gene assay in HepG2 cells showed 100% expression compared to wild-type, reported in PMID 31395865 by Kircher et al, 2019 from University of Washington, Seattle, USA.