The m.5532G>A variant in MT-TW has been reported in one individual with primary mitochondrial disease to date (PMID: 15054399), in a 16-year-old girl with failure to thrive, short stature, recurrent vomiting, developmental delay and regression, ophthalmoplegia, ptosis, pigmentary retinopathy, sensorineural hearing loss, and myopathy. Brain imaging showed generalized atrophy and periventricular white matter changes. Elevated lactate was seen in blood and cerebrospinal fluid. Muscle biopsy showed ragged red fibers and mitochondrial respiratory chain complex I and IV deficiency. The variant was present at 92% heteroplasmy in muscle, 37% in fibroblasts, and 21% in blood. The variant was present at lower heteroplasmy levels in blood from her healthy mother (7%) and brother (9%; PP1; PMID: 15054399). There are no other individuals or families reported in the medical literature. Although there is one occurrence of this variant in population databases (1/61,134 in MITOMAP, absent in Helix and gnomad v3.1.2), the frequency is still low (PM2_supporting). In silico predictors are conflicting as the computational predictor MitoTIP suggests this variant is likely benign (19.4 percentile) but HmtVAR predicts it to be deleterious with a score of 0.7. Single fiber testing showed higher levels of the variant in COX-negative fibers (>90%) than in COX-positive fibers (6-85%, p<0.0001, PS3_supporting, PMID: 15054399). In summary, this variant meets criteria to be classified as uncertain significance for primary mitochondrial disease inherited in a mitochondrial manner. This classification was approved by the NICHD/NINDS U24 ClinGen Mitochondrial Disease Variant Curation Expert Panel on May 13, 2024. Mitochondrial DNA-specific ACMG/AMP criteria applied (PMID: 32906214): PP1, PS3_supporting, PM2_supporting.