The NM_003494.4: c.2779del p.(Ala927LeufsTer21) variant in DYSF, which is also known as NM_001130987.2: c.2833del p.(Ala945LeufsTer21), is a frameshift variant predicted to cause a premature stop codon in biologically relevant exon 27/55 leading to nonsense mediated decay in a gene in which loss of function is an established disease mechanism (PVS1). This variant has been detected in at least 13 individuals with limb girdle muscular dystrophy. Of those individuals, one was compound heterozygous for the variant and a pathogenic variant (c.857T>A (p.Val286Glu), 1.0 pt, PMID: 18832576), and 10 were homozygous (0.75 pts, PMID: 16010686, 17825554) (PM3). At least one patient with this variant displayed progressive limb girdle muscle weakness and absent dysferlin protein expression, which is highly specific for DYSF-associated LGMD (PP4_Strong, PMID: 18832576, 17825554). The variant was also reported to co-segregate with the disease in 10 affected family members and has been described as a founder variant among Jews of the Caucus region, with an estimated carrier frequency of 4% (PP1 (capped with PP4_Strong); PMID: 17825554). The filtering allele frequency of the variant is 0.0006 for Admixed American genome alleles in gnomAD v3.1.2 (the upper threshold of the 95% CI of 4/15288), which is greater than the LGMD VCEP threshold (<0.0001) for PM2_Supporting (criterion not met). In summary, this variant meets the criteria to be classified as Pathogenic for autosomal recessive limb girdle muscular dystrophy based on the ACMG/AMP criteria applied, as specified by the ClinGen LGMD VCEP (LGMD VCEP specifications version 1.0.0; 01/08/2025): PVS1, PM3, PP4_Strong, PP1.