The NM_000070.3: c.883_886delinsCTT p.(Asp295LeufsTer57) variant in CAPN3 is a frameshift variant predicted to cause a premature stop codon in biologically relevant exon 8/24, leading to nonsense mediated decay in a gene in which loss of function is an established disease mechanism (PVS1). This variant has been reported in at least 4 unrelated patients with clinical signs of LGMD (PMID: 16141003, 15733273, 17994539, 16650086, 15689361, 30919934), including in a homozygous state in one individual without reported familial consanguinity (0.5 pts, PMID: 1614100; PM3_Supporting). This variant has also been reported to co-segregate with autosomal recessive LGMD in 3 affected members of a single family (PMID: 16141003; PP1_Moderate). In addition, at least one of the patients with this variant and a second presumed diagnostic CAPN3 variant had a clinical diagnosis of LGMD and absent calpain-3 protein expression in skeletal muscle, which is highly specific for CAPN3-related LGMD (PP4_Moderate, capped with PP1_Moderate; PMID: 16650086, 15689361). This variant is absent from gnomAD v4.1.0 (PM2_Supporting). In summary, this variant meets the criteria to be classified as Pathogenic for autosomal recessive limb girdle muscular dystrophy based on the ACMG/AMP criteria applied, as specified by the ClinGen LGMD VCEP (LGMD VCEP specifications version 1.0.0; 06/03/2025): PVS1, PM3_Supporting, PP1_Moderate, PP4_Moderate, PM2_Supporting.