NM_001034853.2(RPGR):c.389T>C (p.Phe130Ser) is a missense variant predicted to cause substitution of phenylalanine by serine at amino acid 130. This variant is absent from hemizygous individuals in gnomAD v4.1.0 (PM2_Supporting). The computational predictor REVEL gives the variant a score of 0.761, which is between the ClinGen X-linked IRD VCEP thresholds of 0.664 to 0.772 and predicts a damaging effect on RPGR function (PP3). The computational splicing predictor SpliceAI gives a delta score of 0.01 for donor loss, which is below the ClinGen X-linked IRD VCEP threshold of >0.2 and does not predict that the variant disrupts RPGR splicing. At least 1 patient with this variant has been diagnosed with X-linked retinopathy, but phenotype details necessary to meet PP4 or include the patient in PS4_Supporting have not been reported (PMID: 28863407, PMID: 32531858, SCV001162677.1). Another missense variant in the same codon, NM_001034853.2(RPGR):c.389T>G (p.Phe130Cys), has been reported in a patient with X-linked retinopathy (PMID: 8817343). However, the present variant has a lower Grantham score (155) than the comparison variant (205), so that the PM5 code cannot be evaluated. In summary, this variant is classified as a variant of uncertain significance for RPGR-related retinopathy based on the ClinGen X-linked Inherited Retinal Disease Expert Panel Specifications to the ACMG/AMP Variant Interpretation Guidelines for RPGR Version 1.0.0; PM2_Supporting and PP3. (date of approval 05/16/2025).