The NM_000038.6(APC):c.835-7T>G variant in APC is an intronic variant in intron 8. This variant has been reported in 3 families meeting phenotypic criteria, resulting in a total phenotype score of 2.5 (PS4_Moderate, PMID: 18433509, internal data Labcorp Genetics (formerly Invitae)). This variant is absent from gnomAD v2.1.1 (PM2_Supporting). The results from ≥ 2 in silico splicing predictors (SpliceAI and MaxEnt) indicate that this variant may affect splicing by disrupting the acceptor splice site of intron 8 and creating a cryptic acceptor splice site (PP3). RT-PCR sequencing of cDNA demonstrated that the variant impacts splicing by insertion of the last six bases of intron 8 between exon 8 and 9, the second inserted codon is a stop codon. However, relative quantitation of full-length normal transcript versus mutant was not performed (PS3_Moderate, PMID 18433509). In summary, this variant is a Likely Pathogenic variant for autosomal-dominant inherited FAP based on the ACMG/AMP criteria applied, as specified by the ClinGen InSiGHT Hereditary Colorectal Cancer/Polyposis VCEP: PS4_Moderate, PS3_Moderate, PM2_Supporting and PP3 applied (VCEP specifications version v2.1.0; date of approval 11/24/2023).