The NM_000330.4(RS1):c.69G>A variant is a synonymous variant at amino acid 23. The splicing impact predictor SpliceAI gives a delta score of 0.06, which is below the ClinGen X-linked IRD VCEP recommended threshold of <0.1 and does not strongly predict an impact on splicing (BP4). This silent variant c.69G>A causing a synonymous variant at codon 23 does not have an impact at splicing sites according to Splice AI, which predicts a delta score of 0.06 for donor loss which is below the ClinGen X-linked IRD VCEP recommended threshold of <0.2 and does not strongly predict an impact on splicing (BP7). This variant is present in gnomAD v.4.1.0 at a frequency of 0.000007727 among hemizygotes, with 3 variant alleles / 388269 total hemizygous alleles, which is between the ClinGen X-linked IRD VCEP thresholds of >0.00002 and <0.000002 and fails to meet either BS1 or PM2_supporting. In summary, this variant is classified as likely benign for X-linked retinoschisis based on the ClinGen X-linked Inherited Retinal Disease Expert Panel Specifications to the ACMG/AMP Variant Interpretation Guidelines for RS1 Version 1.0.0: BP7 and BP4_supporting. (date of approval 01/24/2025).