NM_005026.5(PIK3CD):c.241G>A (p.Glu81Lys) is a missense variant causing substitution of glutamic acid by lysine at amino acid 81. This variant is present in gnomAD v4.1.0 at a total combined allele frequency of 0.0000006197, with 1 allele / 1,613,806 total alleles across all populations of gnomAD, which is lower than the ClinGen Antibody Deficiencies VCEP PM2_Supporting threshold of <0.00000132 (PM2_Supporting). At least one patient with this variant had a phenotype that included recurrent sinopulmonary infections and otitis media (4 pts), lymphoma (1 pt), lymphadenopathy (4 pts), eczema (1 pt), increased circulating antibody levels (0.5 pts), increased proportion of transitional B cells (2 pts), and lymphopenia (1 pt), with genotyping to rule out an alternative basis for disease in the PIK3R1 gene (13.5 total points, PMID: 28414062). Additionally, a phospho-AKT assay in patient T cells revealed over-activation of the PI3K pathway, which was rescued by inhibition using idelalisib (PMID: 28414062, PP4_Moderate). A second proband has a phenotype including lymphoproliferation with splenomegaly (4 pts), hepatic fibrosis and portal hypertension with gastrointestinal bleeding (1 pt), bronchiectasis (4 pts), lymphopenia (1 pt), and decreased IgG (0.5 pts), with genotyping that excluded an alternative basis for disease in PIK3R1 (10.5 pts, PMID:28428270). The variant was identified as a de novo occurrence with unconfirmed parental relationships in the proband (PS2_Moderate; PMID: 28428270). A kinase activity assay using this PIK3CD variant in healthy (non-patient) T cells showed increased phospho-AKT, indicating that the variant disrupts protein function (PS3_Supporting; PMID: 28414062). The computational predictor REVEL gives a score of 0.661, which is above the ClinGen Antibody Deficiencies VCEP threshold of >0.644 and predicts a damaging effect on PIK3CD function. The computational predictor CADD gives a PHRED score of 31.0, which is above the ClinGen Antibody Deficiencies VCEP threshold of >25.3 and predicts a deleterious effect on PIK3CD function. The two predictors agree on a damaging effect (PP3). In summary, this variant meets the criteria to be classified as likely pathogenic for autosomal dominant immunodeficiency 14 based on the ACMG/AMP criteria applied, as specified by the ClinGen Antibody Deficiencies VCEP: PS2_Moderate, PS4_Supporting, PP4_Moderate, PM2_Supporting, PS3_Supporting, and PP3. (VCEP specifications version 1.0.0).