The NM_000070.3: c.1343G>T variant in CAPN3 is a missense variant predicted to cause the substitution of arginine by leucine at amino acid position 448, p.(Arg448Leu). This variant has been identified in at least six unrelated individuals with features consistent with LGMD (PMID: 25987458, 29797799, 22926650, 33250842, 32994280, 28403181), including in a homozygous state in one patient without reported consanguinity (0.5 pts, PMID: 32994280), confirmed in trans with a pathogenic variant in one patient (c.734dup p.(Ser246Ter), 1.0 pt, PMID: 28403181), and in unconfirmed phase with a pathogenic variant in one patient (c.2120A>G p.(Asp707Gly), 0.5 pts, PMID: 3299428). In two other patients, it was reported in unconfirmed phase with a variant classified as likely pathogenic by the VCEP (c.1319G>A p.(Arg440Gln), 0.25 pts, PMID: 33250842; c.2306G>C p.(Arg769Pro), 0.25 pts, PMID: 25987458) (PM3_Strong). At least one patient with this variant and a second presumed diagnostic CAPN3 allele displayed progressive limb girdle muscle weakness and reduced (<50%) or absent expression of calpain-3 protein in skeletal muscle, though the exact level was not specified (PMID: 22926650; PP4). This variant is absent from gnomAD v.4.1.0, meeting the criteria for PM2_Supporting. The computational predictor REVEL gives a score of 0.872, which is above the LGMD VCEP threshold of ≥0.70, evidence that correlates with impact to CAPN3 function. In addition, another missense variant at the same codon, c.1343G>A (p.Arg448His), has been classified as Pathogenic for autosomal recessive limb girdle muscular dystrophy by the LGMD VCEP (PM5). In summary, this variant meets the criteria to be classified as Likely Pathogenic for autosomal recessive limb girdle muscular dystrophy based on the ACMG/AMP criteria applied, as specified by the ClinGen LGMD VCEP (LGMD VCEP specifications version 2.0.0; 03/12/2026): PM3_Strong, PP4, PM2_Supporting, PP3, PM5.