The c.2278C>T variant in VWF is a missense variant predicted to cause substitution of Arginine by Cysteine at amino acid 760 (p.Arg760Cys). This variant lies 4 amino acids before the cleavage site for pro-VWF. At least 1 patient with this variant displayed excessive mucocutaneous bleeding as well as low FVIII activity and decreased VWF:FVIII binding 0f 0.23, which is highly specific for VWD type 2N. (PP4_moderate, PMID: 12393698). This individual was compound heterozygous (confirmed in trans by parental testing) for this variant and the p.R854Q variant which was classified as pathogenic by the VCEP (1 PM3 points, PM3). Factor VIII binding assay performed with the p.Arg760Cys recombinant mutant and wt vWF expressed by FUR4BHK cells showed absent binding indicating that this variant has a damaging effect on protein function (PMID: 12393698 )(PS3). The Grpmax filtering allele frequency in gnomAD v4.1 is 0.000007410 (based on 2/60034 alleles in the Admixed American population), which is lower than the ClinGen VWD VCEP threshold of <0.005 for type 2N VWD (PM2_Supporting). The computational predictor REVEL gives a score of 0.219, which is below the ClinGen VWD VCEP threshold of <0.290 and does not predict a damaging effect on VWF function. Additionally, the computational splicing predictor SpliceAI indicated that the variant has no impact on splicing (BP4). In summary, this variant meets the criteria to be classified as Likely Pathogenic for autosomal recessive von Willebrand disease type 2N based on the ACMG/AMP criteria applied, as specified by the ClinGen VWD VCEP: PP4_Moderate, PM2_Supporting, PM3, PS3, and BP4 (VCEP specifications version 1.0.0; date of approval).