NM_000314.8(PTEN):c.10A>G (p.Ile4Val) is currently classified as a variant of uncertain significance for PTEN Hamartoma Tumor syndrome in an autosomal dominant manner using modified ACMG criteria (ACMG Classification Rules Specified for PTEN Variant Curation version 3.0.0). Please see a summary of the rules and criteria codes in the “PTEN ACMG Specifications Summary” document (assertion method column). PS2: De novo (both maternity and paternity confirmed) observation in a patient with the disease and no family history (SCV002569820.2). PM2_Supporting: Absent in large sequenced populations OR present at extremely low (<0.00001, 0.001%) allele frequency in the gnomAD cohort. (PMID 27535533). PP2: PTEN is defined by the PTEN Expert Panel as a gene that has a low rate of benign missense variation and where missense variants are a common mechanism of disease. BS3_Supporting: Well-established functional studies show no deleterious effect: Phosphatase activity ≤ -1.11 (score = 1.00) per Mighell et al. 2018 (PMID: 29706350). BP4: REVEL score < 0.5 (score=0.294) Using the Bayesian point system (PMID: 29300386) for this variant with conflicting evidence: 1 pathogenic strong, 2 pathogenic supporting and 2 benign supporting codes get 4 + (1*2) +(- 1*2) points = total is 4 (VUS).