The NM_000329.3(RPE65):c.762G>T (p.Glu254Asp) variant is a missense variant in RPE65 causing a substitution of glutamic acid with aspartic acid at position 254. This variant is absent from gnomAD v4.1.0 (PM2_Supporting). The computational predictor REVEL gives a score of 0.774, which is above the ClinGen LCA/eoRD VCEP threshold of ≥0.773 and predicts a damaging effect on RPE65 function (PP3_Moderate). This variant has been reported in at least 3 unrelated probands with early-onset severe retinal dystrophy who were homozygous for the variant (1 point, PM3, PMID: 35129589). At least one proband harboring this variant exhibits a phenotype including clinical diagnosis of LCA (0.5 pt) and significant, documented improvement of FST or other measure of dark-adapted vision after treatment with Luxturna or other RPE65 gene therapy. (8.5 points, PMID: 38295874, PP4_Moderate). In summary, this variant meets the criteria to be classified as Likely Pathogenic for RPE65-related recessive retinopathy based on the ACMG/AMP criteria applied, as specified by the ClinGen LCA/eoRD VCEP: PM2_Supporting, PP3_Moderate, PP4_Moderate, PM3 (VCEP specifications version 1.0.0; date of approval 09/21/2023).