The c.1317C>T variant in MYOC is a synonymous variant (p.Val439=). The highest minor allele frequency of this variant was in the European (Finnish) population of gnomAD (v2.1.1) = 0.003065, which met the ≥ 0.001 threshold set for BS1 (77 alleles out of 25 124, meeting the threshold of ≥ 5 of at least 2,000 observed alleles). The CADD score (v1.6) = 4.154, which met the ≤ 10 threshold for BP4 and this variant was not predicted to affect splicing, as assessed with SpliceAI (≤ 0.2), suggesting that the variant does not impact MYOC function. However, BP7 was not met as this variant had a GERP score = 2.14 (threshold <0), indicating conservation at this site. There was no functional evidence predicting a damaging or benign impact of this variant on MYOC function. As PM2_Supporting was not met PS4 did not apply. Additionally, this variant has not yet been identified in a proband with juvenile or primary open angle glaucoma, only in an individual with exfoliative glaucoma. In summary, this variant met the criteria to receive a score of -5 and to be classified as likely benign (likely benign classification range -2 to -6) for primary open angle glaucoma based on the ACMG/AMP criteria met, as specified by the ClinGen Glaucoma VCEP (v1, 12 Oct 2021): BS1, BP4