The c.1001T>C variant in MYOC is a missense variant predicted to cause substitution of Leucine by Proline at amino acid 334 (p.Leu334Pro). The highest minor allele frequency of this variant was in the Latino/Admixed American population of gnomAD (v2.1.1) = 0.00002891 (1 allele out of 34,592), which met the ≤ 0.0001 threshold set for PM2_Supporting in a population of at least 10,000 alleles. The REVEL score = 0.936, which met the ≥ 0.7 threshold for PP3, predicting a damaging effect on MYOC function. There was no functional evidence predicting a damaging or benign impact of this variant on MYOC function. 3 segregations in 1 family, with juvenile open angle glaucoma (JOAG), have been reported (PMID: 18385784), which fulfilled PP1 (3-4 meioses). Only 1 proband with JOAG had been reported (PMID: 18385784), not meeting the ≥ 2 probands threshold required to meet PS4_Supporting. In summary, this variant met the criteria to receive a score of 3 and to be classified as a variant of uncertain significance (uncertain significance classification range -1 to 5) for juvenile open angle glaucoma based on the ACMG/AMP criteria met, as specified by the ClinGen Glaucoma VCEP (v1, 12 Oct 2021): PP1, PP3, PM2_Supporting.