The c.985G>A variant in MYOC is a missense variant predicted to cause substitution of Valine by Methionine at amino acid 329 (p.Val329Met). The highest minor allele frequency of this variant was in the African/African-American population of gnomAD (v2.1.1) = 0.002523, which met the ≥ 0.001 threshold set for BS1 (63 alleles out of 24,968, meeting the threshold of ≥ 5 of at least 2,000 observed alleles). The REVEL score = 0.466, which was neither above nor below the thresholds for PP3 (≥ 0.7) or BP4 (≤ 0.15), predicting a damaging or benign impact on MYOC function. A previous study (PMID: 11004290) demonstrated that the Val329Met protein had similar solubility levels to wild type myocilin protein and met the OddsPath threshold for BS3_Moderate (< 0.23), indicating that this variant did not impact protein function. Although probands with juvenile or primary open angle glaucoma have been reported carrying this variant, PM2_Supporting was not met, therefore PS4 did not apply. In summary, this variant met the criteria to receive a score of -6 and to be classified as likely benign (likely benign classification range -2 to -6) for juvenile open angle glaucoma based on the ACMG/AMP criteria met, as specified by the ClinGen Glaucoma VCEP (v1, 12 Oct 2021): BS1, BS3_Moderate.