The c.4916G>C variant in SCN1A is a missense variant predicted to cause substitution of arginine by proline at amino acid 1639 (p.Arg1639Pro). This variant has been reported in at least one individual with Dravet syndrome and another individual with epilepsy with no additional details (PMIDs:35087721, 29655203) (PS4_Moderate). Multiple missense variants in SCN1A and in paralogous genes have previously been reported and meet P/LP per these criteria, including SCN1A: p.R1639G (PMIDs:18930999), SCN2A: p.R1629L, p.R1629H and SCN8A: p.R1620L (PMIDs 28330790, 3061509). (PM5_Strong). An identical amino acid substitution at the corresponding position in the paralogous gene, SCN2A has been reported as de novo (p.R1629P), however this did not meet P/LP per these criteria so PS1 was not met. This variant is absent from the population database, gnomAD v2.1.1 (PM2_Supporting). It falls within a pathogenic enriched region that is defined as a mutational hotspot (PM1). The computational predictor REVEL gives a score of 0.98, which is above the threshold of 0.773, evidence that correlates with a maximum strength of PP3_Moderate. In summary, this variant meets the criteria to be classified as pathogenic for autosomal dominant Dravet syndrome based on the ACMG/AMP criteria applied, as specified by the ClinGen Epilepsy Sodium Channel VCEP: PS4_Moderate, PM5_Strong, PM1, PM2_Supporting, PP3_Moderate (version 1.0; 5/9/23).