The c.856T>C variant in MYOC is a missense variant predicted to cause substitution of Tryptophan by Arginine at amino acid 286 (p.Trp286Arg). The highest minor allele frequency of this variant was in the Latino/Admixed American population of gnomAD (v2.1.1) = 0.00005811 (2 alleles out of 34,418), which met the ≤ 0.0001 threshold set for PM2_Supporting in a population of at least 10,000 alleles. The REVEL score = 0.917, which met the ≥ 0.7 threshold for PP3, predicting a damaging effect on MYOC function. A previous study (PMID: 16466712) demonstrated that the Trp286Arg protein had reduced secretion levels compared to wild type myocilin protein and met the OddsPath threshold for PS3_Moderate (> 4.3), indicating that this variant did impact protein function. Only 2 segregations had been reported for primary open angle glaucoma (E. Souzeau pers. comm.), not meeting the ≥ 3 segregations required for PP1. 5 probands with juvenile or primary open angle glaucoma have been reported carrying this variant (PMIDs: 23453510, 22879734, 9535666, E. Souzeau pers. comm.), which met PS4_Supporting (≥ 2 probands). In summary, this variant met the criteria to receive a score of 5 and to be classified as a variant of uncertain significance (uncertain significance classification range -1 to 5) for juvenile open angle glaucoma based on the ACMG/AMP criteria met, as specified by the ClinGen Glaucoma VCEP (v1, 12 Oct 2021): PS3_Moderate, PP3, PS4_Supporting, PM2_Supporting.